Technical Reference #999

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
coverslip-bottomed dishes (MatTek Cultureware; Ashland; MA)

999.

Fcr-Stimulated Activation Of The Nadph Oxidase: Phosphoinositide-Binding Protein P40Phox Regulates Nadph Oxidase Activity After Enzyme Assembly On The Phagosome Wei Tian; Xing Jun Li; Natalie D. Stull; Wenyu Ming; Chang-Il Suh; Sarah A. Bissonnette; Michael B. Yaffe; Sergio Grinstein; Simon J. Atkinson; and Mary C. Dinauer, Indiana University, Blood, 112(999), (2008)
Link To Paper

Abstract:
The phagocyte NADPH oxidase generates superoxide for microbial killing and includes a membrane-bound flavocytochrome b558 and cytosolic p67phox p47phox and p40phox subunits that undergo membrane translocation upon cellular activation.

Materials & Methods:
COSPF-p67YFP COSPF40-p67YFP and COS7-WF cells were plated into coverslip-bottomed dishes (MatTek Cultureware Ashland MA)

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
COS-PF-p67YFP, COS7-WF