Technical Reference #328

328.

Overexpression Of Mitochondrial Manganese Superoxide Dismutase Protects Against Radiation-Induced Cell Death In The Human Hepatocellular Carcinoma Cell Line Hle Motoori6; S.; Majima; H.; Ebara4; M.; Kato; H.; Hirai; 4; F.; Kakinuma; S.; Yamaguchi; C.; Ozawa; T.; Nagano7; T.; Tsujii; H.; and Saisho4; H.; Research Center of Charged Particle Therapy; International Space Radiation Laboratory; Redox Resear, Chiba University School of Medicine, Cancer Research, 61(328), (2001)
Link To Paper

Abstract:
We investigated the potential role of mitochondrial manganese superoxide dismutase (Mn-SOD) in protective activity against irradiation by analyzing cell viability by a colony formation assay and by detecting apoptosis in stably human Mn-SOD gene-transfect

Materials & Methods:
"Cells grown on glass-bottomed (35-mm) dishes (MatTek Corp. Ashland MA) were stained with Hoechst 33342 fluorescent dye (Molecular Probes Eugene OR). … Glass-bottomed (35-mm) dishes (MatTek Corp.) with monolayers were prepared for staining with DAF-FM

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEP