Technical Reference #325

325.

Identification And Testing Of A Gene Expression Signature Of Invasive Carcinoma Cells Within Primary Mammary Tumors Wang; W.; Goswami; S.; Lapidus; K.; Wells; AL.; Wyckoff; JB.; Sahai; E.; Singer; RH.; Segall; JE.; Condeelis; JS. Department of Anatomy and Structural Biology; Albert Einstein College of Medicine; Bronx; NY; USA. Cancer Research; 64; (); 8585-8594; (00, Albert Einstein College of Medicine of Yeshiva University, Cancer Research, 64(325), (2004)
Link To Paper

Abstract:
We subjected cells collected using an in vivo invasion assay to cDNAmicroarray analysis to identify the gene expression profile of invasivecarcinoma cells in primary mammary tumors. Expression of genes involvedin cell division survival and cell mo

Materials & Methods:
The resulting cells were split and plated on Mattek dishes covered with Matrigel (1:5) in the presence or absence of EGF (1 nmol/L) for 4 hours at 37°C. The cells were then lysed directly on the dish for total RNA extraction.

Cell Type(s)
MTLn3