Technical Reference #323

323.

Measurement Of Barbed Ends; Actin Polymerization; And Motility In Live Carcinoma Cells After Growth Factor Stimulation Lorenz; M.; DesMarais; V.; Macaluso; F.; Singer; RH.; Condeelis; J. Albert Einstein College of Medicine; Department of Anatomy and Structural Biology; Bronx; NY; USA. Cell Motility and the Cytoskeleton; 57; (4); 07-7; (004)., Albert Einstein College of Medicine of Yeshiva University, Cell Motility and the Cytoskeleton, 57(323), (2004)
Link To Paper

Abstract:
Motility is associated with the ability to extend F-actin-rich protrusions anddepends on free barbed ends as new actin polymerization sites. To understand thefunction and regulation of different proteins involved in the process of generatingbarbed e

Keywords:
cytoskeleton; cofilin; fluorescence; GFP; microscopy

Materials & Methods:
GFP-actin-expressing MTLn3 cells were plated on tissue culture dishes containing a coverslip (MatTek Corp.) for 24 h and serum starved for approximately 3 h before EGF stimulation.

Microscopic Technique
Electron Microscopy, Microinjection

Cell Type(s)
Metastatic