Technical Reference #321

321.

Coordination Of Mesangial Cell Contraction By Gap Junction–Mediated Intercellular Ca2+ Wave Yao; J.; Morioka; T.; Li; B.; and Oite; T.; Department of Cellular Physiology; Institute of Nephrology; Niigata University; Niigata; Japan. Journal of American Society of Nephrology; ; (8); 08-06; (00)., Niigata University, Journal of the American Society of Nephrology, 13(321), (2002)
Link To Paper

Abstract:
Gap junction intercellular communication (GJIC) plays a fundamental role in mediating intercellular signals and coordinating multicellular behavior in various tissues and organs. Glomerular mesangial cells (MC) are rich in GJ but the functional associati

Materials & Methods:
MC were cultured to confluence in special glass-bottom microwell dishes (MatTek Corporation Ashland MA) and then loaded with fura-2 by incubation with 5 µmol/L Fura-2 AM in Hanks balanced salt solution (HBSS) containing 2.0 mmol/L CaCl2 and 1 mmol/L MgC

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Glomerular mesangial