Technical Reference #317

317.

The Zinc Transporter Znt3 Interacts With Ap-3 And It Is Preferentially Targeted To A Distinct Synaptic Vesicle Subpopulation Salazar; G.; Love; R.; Werner; E.; Doucette; MM.; Cheng; S.; Levey; ; A.; Faundez; ; V. Department of Cell Biology; Emory University; Atlanta; GA; Department of Neurology; Emory University; Atlanta; GA; and The Center for Neurodegenerative, Emory University, Molecular Biology of the Cell, 15(317), (2004)
Link To Paper

Abstract:
Synaptic vesicles (SV) are generated by two different mechanisms one AP-2 dependent and one AP-3 dependent. It has been uncertain however whether these mechanisms generate SV that differ in molecular composition. We explored this hypothesis by analyzin

Materials & Methods:
Briefly hippocampi from 1- to 4-day postnatal rats were enzymatically digested and plated onto either 10-mm #0 MatTek dishes or 15-mm #0 Deckglaser German glass coverslips coated with Matrigel.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
PC12