Technical Reference #316

316.

Translated Alu Sequence Determines Nuclear Localization Of A Novel Catalytic Subunit Of Casein Kinase 2 Hilgard P.; Huang T.; Wolkoff; ; ; AW.; Stockert; ; RJ. Departments of Medicine and Anatomy and Structural Biology and Marion Bessin Liver Research Center; Albert Einstein College of Medicine; Bronx; NY; USA. Am J Physiol Cell Physiology;, Albert Einstein College of Medicine of Yeshiva University, American Journal of Physiology: Cell Physiology, 283(316), (2002)
Link To Paper

Abstract:
Casein kinase 2 (CK2) is a tetrameric enzyme constitutively expressed in all eukaryotic tissues. The two known isoforms of the catalytic subunit CK2 and CK2' have been reported to have distinct tissue-dependent subcellular distributions. We recently des

Keywords:
nuclear matrix association; human hepatoma cell line HuH-7; casein kinase 2-green fluorescent protein fusion protein

Materials & Methods:
...After 36–40 h cells were trypsinized and replated on MatTek culture dishes containing a 0.17-mm coverslip in the bottom (MatTek Ashland MA).... ...Twenty-four hours after transfection cells were trypsinized and replated on 35-mm MatTek dishes.…

Microscopic Technique
Phase Contrast Microscopy

Cell Type(s)
HeLa