Technical Reference #314

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm glass bottom dishes (MatTek Corporation)

314.

Identification Of Pkczetaii: An Endogenous Inhibitor Of Cell Polarity Parkinson; S.; Le Good; J. A.; Whelan; R.; Whitehead P.; and Parker; P.; Protein Phosphorylation Laboratory; Cancer Research UK; London Research Institute; London; United Kingdom; Dana-Farber Cancer Institute; Division of Cancer Biology; Harvard, London Research Institute, European Molecular Biology Organization Journal, 23(314), (2004)
Link To Paper

Abstract:
A new member of the atypical protein kinase C (aPKC) family designated PKCzetaII is identified in this study. The gene contains no introns and is 98% homologous with the cDNA encoding PKCzeta. The PKCzetaII coding region is frame-shifted with respect to

Keywords:
cell polarity; Par6; PKC; RNAi; tight junction

Materials & Methods:
For live cell microscopy cells were seeded on 35 mm glass bottom dishes (MatTek Corporation) and transfected with the indicated plamids the following day.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-7