Technical Reference #313

313.

Conventional Kinesin Kif5B Mediates Insulin-Stimulated Glut4 Movements On Microtubules Semiz; S.; Park; J.; Nicoloro; S.; Furcinitti; P.; Zhang; C.; Chawla; A.; Leszyk; J.; and Czech; M.; Program in Molecular Medicine; University of Massachusetts Medical School; Worcester; MA; USA. EMBO Journal; ; (0); 87-99; (00)., University of Massachusetts, European Molecular Biology Organization Journal, 22(313), (2003)
Link To Paper

Abstract:
Insulin stimulates glucose uptake in muscle and adipose cells by mobilizing intracellular membrane vesicles containing GLUT4 glucose transporter proteins to the plasma membrane. Here we show in live cultured adipocytes that intracellular membranes contain

Keywords:
adipocytes; GLUT4; insulin; kinesin; microtubules

Materials & Methods:
3T3-L1 adipocytes expressing appropriate cDNA constructs were seeded in glass-bottomed dishes (MatTek Corporation).

Microscopic Technique
Inverted Microscopy

Cell Type(s)
3T3