Technical Reference #312

312.

Nfat Functions As A Working Memory Of Ca2+ Signals In Decoding Ca2+ Oscillation Tomida; T.; Hirose; K.; Takizawa; A.; Shibasaki F.; and Iino; M.; Department of Pharmacology; Graduate School of Medicine; The University of Tokyo; Tokyo and Department of Molecular Cell Physiology; The Tokyo Metropolitan Institute of Medical Science;, The University of Tokyo, European Molecular Biology Organization Journal, 22(312), (2003)
Link To Paper

Abstract:
Transcription by the nuclear factor of activated T cells (NFAT) is regulated by the frequency of Ca2+ oscillation. However why and how Ca2+ oscillation regulates NFAT activity remain elusive. NFAT is dephosphorylated by Ca2+-dependent phosphatase calcine

Keywords:
calcineurin; Ca2+ oscillation; frequency dependence; NFAT; nuclear translocation

Materials & Methods:
"GFP–NFAT expressing BHK cells on poly-D-lysine-coated glass-bottomed dish (MatTek Ashland MA) were loaded at room temperature with 5 µM Fura-PE3AM (TefLabs Austin TX) in physiological salt solution (PSS) containing 150 mM NaCl 4 mM KCl 2 mM CaCl2

Microscopic Technique
Inverted Microscopy

Cell Type(s)
BHK