Technical Reference #309

309.

A Novel Form Of Cellular Communication Among Thymic Epithelial Cells: Intercellular Calcium Wave Propagation Nihei;;; O.; Campos de Carvalho; A.; Spray; D.; Savino;;4 W.; and Alves L.; Laboratory on Thymus Research; Department of Immunology; Institute Oswaldo Cruz; The Oswaldo Cruz Foundation; Rio de Janeiro; Institute of Biophysics; Federal University, The Oswaldo Cruz Foundation, American Journal of Physiology: Cell Physiology, 285(309), (2003)
Link To Paper

Abstract:
We here describe intercellular calcium waves as a novel form of cellular communication among thymic epithelial cells. We first characterized the mechanical induction of intercellular calcium waves in different thymic epithelial cell preparations: cortical

Keywords:
gap junctions; connexin43; P2 receptors • intercellular communication

Materials & Methods:
"The typically heavier TNCs were allowed to sediment. TNCs obtained by this process were cultured for RNA extraction or alternatively plated in MatTek (Ashland MA) imaging dishes for calcium wave experiments."

Microscopic Technique
Confocal Microscopy

Cell Type(s)
TNC