Technical Reference #308

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed dishes (MatTek; Ashland; MA)

308.

Role Of Clathrin- And Actin-Dependent Endocytotic Pathways In Lung Phospholipid Uptake Rückert; P.; Bates; S.; and Fisher; A.; Institute for Environmental Medicine; University of Pennsylvania School of Medicine; Philadelphia; Pennsylvania. Am J Physiol Lung Cell Mol Physiology 84; (6); L98-L989; (00)., University of Pennsylvania, American Journal of Physiology: Lung Cellular and Molecular Physiology, 284(308), (2003)
Link To Paper

Abstract:
We evaluated the contribution of endocytotic pathways to pulmonary uptake of surfactant lipids from the alveolar space. Resting and stimulated 8-bromoadenosine 3'5'-cyclic monophosphate (8-Br-cAMP) uptake of unilamellar liposomes labeled with either [3H]

Keywords:
liposomes; lamellar bodies; alveolar type II cells; perfused rat lung; 1-palmitoyl-2-[12-(7-nitro-2-1;3-benzoxadiazol-4-yl) amino] dodecanoyl-phosphatidylcholine

Materials & Methods:
The cells were cultured for 24 h on 35-mm glass-bottomed dishes (MatTek Ashland MA) in minimal essential medium containing 10% fetal calf serum in 5% CO2 in air at 37°C.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
ALVEOLAR TYPE II