Technical Reference #307

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysinecoated 35-mm glass-bottomed dishes (MatTek; Ashland; MA)

307.

Ampa Receptor-Mediated Miniature Synaptic Calcium Transients In Glur2 Null Mice Wang; S.; Jia; Z.; Roder; J.; and Murphy; T. Kinsmen Laboratory; Department of Psychiatry; University of British Columbia; Vancouver; Program in Brain and Behavior; The Hospital For Sick Children; Toronto; and Samuel Lunenfeld Research Institute, University of British Columbia, Journal of Neurophysiology, 88(307), (2002)
Link To Paper

Abstract:
Fast excitatory synaptic transmission in the CNS is mediatedby -amino-3-hydroxy-5-methyl-4-isoxazlepropionate receptors(AMPARs) and N-methyl-D-aspartate type glutamate receptors(NMDARs). The AMPARs are assembled of four tofive homologous subunits

Materials & Methods:
"... and seeded (2 ml per dish; density = about 1.2 million cells/ml) into poly-D-lysinecoated 35-mm glass-bottomed dishes (MatTek Ashland MA)."

Microscopic Technique
Wide-field Microscopy, Inverted microscope

Cell Type(s)
glial