Technical Reference #305

305.

Ectopic Expression Of The Amino-Terminal Peptide Of Androgen Receptor Leads To Androgen Receptor Dysfunction And Inhibition Of Androgen Receptor-Mediated Prostate Cancer Growth Minamiguchi; K.; Kawada M.; Ohba S.; Takamoto K.; Ishizuka M. Institute for Chemotherapy; Microbial Chemistry Research Foundation; Numazu; Shizuoka; Japan. Hanno Research Center; Taiho Pharmaceutical Co. Ltd; Hanno; Saitama; Japan. Molecular an, Microbial Chemistry Research Foundation, Molecular and Cellular Endocrinology, 214(305), (2004)

Abstract:
Androgen receptor (AR) is a ligand-activated transcription factor that requires androgen binding to initiate a series of molecular events leading to specific gene activation. AR has been suggested to form an antiparallel homodimer based on the characteris

Keywords:
Androgen receptor; Amino- to carboxyl-terminal interaction; Prostate cancer; Amino-terminal peptide; Growth inhibition

Materials & Methods:
The cells were divided into 35-mm glass-bottom dishes (MatTek Corporation) and then were transfected with 0.5 mug of the pEGFP-AR and 2 mug of the pTriARN34 or 2 mug of the empty vector…

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Prostate