Technical Reference #303

303.

Electroporation Loading And Photoactivation Of Caged Insp3: Tools To Investigate The Relation Between Cellular Atp Release In Response To Intracellular Insp3 Elevation Braet; K.; Mabilde; C.; Cabooter; L.; Rapp; G.; Leybaert; L. Physiology and Pathophysiology; Ghent University; Ghent; Belgium. Rapp OptoElectronic GmbH; Hamburg; Germany. Journal of Neuroscience Methods; ; (); 8-89; (004)., Ghent University, The Journal of Neuroscience Methods, 132(303), (2004)

Abstract:
Photolytic liberation of InsP3 in single cells triggers cell-to-cell propagating calcium changes that are communicated by a gap junctional and a paracrine purinergic pathway involving InsP3-triggered ATP release. We investigated the relation between the I

Keywords:
Flash photolysis; Electroporation; Caged compounds; InsP3; ATP release; Calcium signaling; Calcium waves

Materials & Methods:
...this study we used ECV304 cells (European Collection of Animal Cell Cultures Salisbury UK) grown on glass bottom microwells (MatTek Corporation Ashwood MA USA). Cultures were maintained in Medium-199 (Gibco Merelbeke Belgium) with 10% fetal bovi

Cell Type(s)
ECV304