Technical Reference #208
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
glassbottom microwell dishes (MatTek Corp.; Ashland; Mass.) 
208. |
An Avian Sarcoma/Leukosis Virus-Based Gene Trap Vector for Mammalian Cells
Xiangqun H. Zheng; Stephen H. Hughes,
NCI-Frederick Cancer Research and Development Center,
The Journal of Virology,
73(208),
(1999)
Link To Paper
Abstract:
RCASBP-M2C is a retroviral vector derived from an avian sarcoma/leukosis virus which has been modifiedso that it uses the envelope gene from an amphotropic murine leukemia virus (E. V. Barsov and S. H. HughesJ. Virol. 70:3922–3929 1996). The vector Materials & Methods:
Plasmid construction. pGT-GFP (Fig. 1) was derived from the vector
RCASBP-M2C (5). A gfp cassette containing a splice acceptor the GFP coding
sequence preceded by a 14-codon linker and a unidirectional simian virus 40
(SV40) polyadenylation site was inserted at the end of the env gene in RCASBPM2C.
The gfp cassette was placed in the RCASBP-M2C vector in an orientation
opposite to the viral genes. The gfp gene in the cassette was derived from the Microscopic Technique
Confocal Microscopy Cell Type(s)
Embryos |
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