Technical Reference #1799

1799.

Attenuation of Canonical Transient Receptor Potential-Like Channel 6 Expression Specifically Reduces the Diacylglycerol-Mediated Increase in Intracellular Calcium in Human Myometrial Cells Daesuk Chung; Yoon-Sun Kim; Jennifer N. Phillips; Aida Ulloa; Chun-Ying Ku; Henry L. Galan; Barbara M. Sanborn, Colorado State University University of Colorado Health Sciences Center, Endocrinology, 151(1799), (2010)
Link To Paper

Materials & Methods:
Fura 2-acetoxymethylester (AM) and pluronic acid F127 were obtained from Invitrogen (Carlsbad CA). Thapsigargin oxytocin nifedipine and other compounds were purchased from Sigma-Aldrich (St. Louis MO). OAG was obtained from Calbiochem (La Jolla CA). Cell culture medium and other tissue culture reagents were obtained from Invitrogen/Life Technologies Inc. (Carlsbad CA). Oligonucleotides were purchased from Integrated DNA Technologies Inc. (Coralville IA). Restriction enzymes were purchased from New England Biolabs (Ipswich MA). A7r5 cells were obtained from American Type Culture Collection (Manassas VA). Uterine smooth muscle cells (UtSMCs) derived from nonpregnant human myometrium (catalog no. CC-2562 lot no. 17590) were purchased from Lonza (Walkersville MD).

Microscopic Technique
InCyt2 imaging system

Cell Type(s)
UtSMCs