Technical Reference #1739

1739.

Autophagy and Protein Kinase C Are Required for Cardioprotection by Sulfaphenazole Chengqun Huang; Wayne Liu; Cynthia N. Perry; Smadar Yitzhaki; Youngil Lee; Hua Yuan; Yayoi Tetsuo Tsukada; Anne Hamacher-Brady; Robert M Mentzer; Jr.; Roberta A. Gottlieb, San Diego State University, American Journal of Physiology - Heart and Circulatory Physiology, 298(1739), (2010)
Link To Paper

Materials & Methods:
Langendorff perfusion. The isolated perfused rat heart model was utilized as previously described (8 9). In brief after anesthesia and heparinization (pentobarbital sodium 60 mg/kg i.p. and heparin 500 U i.p.) rat hearts were excised into ice cold Krebs-Henseleit solution (mM 118.5 NaCl 4.7 KCl 1.18 KH2PO4 1.18 MgSO4 25 NaHCO3 11.1 glucose 2.5 CaCl2 ) and perfused with xygenated buffer within 30 s. Hearts were perfused at constant pressure (60 mm Hg) for 5 min before administration of any drugs. Where indicated sulfaphenazole dissolved in dimethyl sulfoxide (SUL 10 μM) was administered throughout the perfusion. For hemodynamic analysis a balloon made by plastic wrap was inserted into the ventricle through the left atrium. Hemodynamic parameters were recorded with the EMKA system. All procedures were approved by the Animal Care and Use Committee at The Scripps Research Institute and at San Diego State University and conform to the Guide for the Care and Use of Laboratory Animals (National Institutes of Health publication no. 85-23 revised 1996).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Adult rat cardiomyocytes