Technical Reference #163

163.

Zinc induces a Src family kinase-mediated up-regulation of NMDA receptor activity and excitotoxicity Pat Manzerra; M. Margarita Behrens; Lorella M. T. Canzoniero; Xue Qing Wang; Vale´ rie Heidinger; Tomomi Ichinose; Shan Ping Yu; and Dennis W. Choi, Washington School of Medicine, PNAS, 98(163), (2001)

Abstract:
Zinc is coreleased with glutamate from excitatory nerve terminals throughout the central nervous system and acutely inhibits Nmethyl- D-aspartate (NMDA) receptor activation. Here we report that cultured murine cortical neurons briefly exposed to sublethal

Materials & Methods:
Mixed or near-pure neuronal cortical cell cultures were prepared from fetal mice (15–16 days gestation) as previously described (51). Dissociated cortical cells were plated on established glial cultures in 24-well culture plates or in glass-bottom 35-mm dishes (MatTek Ashland MA) or for

Microscopic Technique
bright-field microscopy

Cell Type(s)
Mammalian, Dissociated cortical cells