Technical Reference #1548

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
uncoated glass-bottom microwells (MatTek Corporation; Ashland; MA)

1548.

Calcium Homeostasis And Reactive Oxygen Species Production In Cells Transformed By Mitochondria From Individuals With Sporadic Alzheimer'S Disease Jason P. Sheehan; Russel H. Swerdlow; Scott W. Miller; Robert E. Davis; Jan K. Parks; W. Davis Parker; Jeremy B. Tuttle, University of Virginia, The The Journal of Neuroscience, 17(1548), (1997)
Link To Paper

Abstract:
Alzheimer's disease (AD) is associated with defects in mitochondrial function. Mitochondrial-based disturbances in calcium homeostasis reactive oxygen species (ROS) generation and amyloid metabolism have been implicated in the pathophysiology of sporadi

Keywords:
Alzheimer's disease; mitochondria; calcium; neurodegeneration; reactive oxygen species; nerve growth factor; beta-amyloid

Materials & Methods:
ATP ADP and AMP standards for HPLC were prepared from Sigma (St. Louis MO) reagents. A dimethylsulfoxide (DMSO) stock of CCCP (10 mM; Sigma) was used. The dilution factor for the DMSO stock was 1:1000.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
SH-SY5Y