Technical Reference #1519

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35mm dishes fitted with polylysine-coated glass coverslips (MatTek Corp; Ashland; MA; USA)

1519.

Ccaat/Enhancer Binding Protein Beta (C/Ebp[Beta])-2 Transforms Normal Mammary Epithelial Cells And Induces Epithelial To Mesenchymal Transition In Culture L.M. Bundy; L. Sealy, Vanderbilt University, Oncogene, 22(1519), (2003)
Link To Paper

Abstract:
The transcription factor CCAAT/enhancer binding protein(C/EBP)b is critical for normal growth anddifferentiation of the mammary gland.The intronlessC/EBPb gene encodes a single mRNA that produces threeprotein isoforms C/EBPb-1 -2 and -3 which

Keywords:
C/EBP transcription factor; anchorage independence; EMT; invasion; mammary gland; retroviral vector

Materials & Methods:
Tissue culture media obtained from Life Technologies Inc. (Gaithersburg MD USA). Unless otherwise indicated all tissue culture supplements were purchased from Sigma Chemical Co. (St Louis MO USA). The antibodies directed against C/EBPb

Microscopic Technique
Fluorescence Microscopy, Epifluorescence Microscopy

Cell Type(s)
MCF10A