Technical Reference #1489

1489.

Adenosine A2A Receptor Dynamics Studied With The Novel Fluorescent Agonist Alexa488-Apec Frank Brand; Athena M. Klutz; Kenneth A. Jacobson; Bertil B. Fredholm; Gunnar Schulte, Karolinska Institutet, European Journal of Pharmacology, 590(1489), (2008)
Link To Paper

Abstract:
G protein-coupled receptors such as the adenosine A2A receptor are dynamic proteins which undergo agonistdependentredistribution from the cell surface to intracellular membranous compartments such as endosomes.In order to study the kinetics of ade

Keywords:
G protein-coupled receptor Endocytosis Cyclic AMP CREB Nucleoside Rab5 Clathrin

Materials & Methods:
For live-cell imaging or immunocytochemistry cells were seeded on collagen-coated (0.1% solution Sigma-Aldrich St. Louis MO USA) glass bottom (MatTek corporation Ashland MA USA) dishes or coverslips respectively.

Microscopic Technique
Live Cell Microscopy

Cell Type(s)
HEK-293, CHO