Technical Reference #1459

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm dishes (MatTek; Ashland; MA)

1459.

The Role Of Grasp55 In Golgi Fragmentation And Entry Of Cells Into Mitosis Juan Manuel Duran; Matt Kinseth; Carine Bossard; David W. Rose; Roman Polishchuk; Christine C. Wu; John Yates; Timo Zimmerman; and Vivek Malhotra, Centro de Regulacion Genomica, Molecular Biology of the Cell, 19(1459), (2008)
Link To Paper

Abstract:
GRASP55 is a Golgi-associated protein but its function at the Golgi remains unclear. Addition of full-length GRASP55 GRASP55-specific peptides or an anti-GRASP55 antibody inhibited Golgi fragmentation by mitotic extracts in vitro and entry of cells in

Keywords:
GRASP55; Golgi; Golgi fragmentation; mitosis

Materials & Methods:
Forty-eight hours after transfection with siRNA the cells were plated in 35-mm dishes (MatTek Ashland MA).

Microscopic Technique
Fluorescence Microscopy, Fluorescence Redistribution After Photobleaching

Cell Type(s)
HeLa