Technical Reference #1447

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-d-lysine-coated coverslips (MatTek)

1447.

New Determinant For The Cavβ2 Subunit Modulation Of The Cav1.2 Calcium Channel Qi Zong Lao; Evgeny Kobrinsky; Jo Beth Harry; Arippa Ravindran; and Nikolai M. Soldatov, National Institutes of Health, The Journal of Biological Chemistry, 283(1447), (2008)
Link To Paper

Abstract:
Cavβ subunits support voltage gating of Cav1.2 calcium channels and play important role in excitation-contraction coupling. The common central membrane-associated guanylate kinase (MAGUK) region of Cavβ binds to the -interaction domain (AID) and the IQ mo

Keywords:
calcium channels; MAGUK

Materials & Methods:
Electrophysiology: COS1 cells were grown on poly-D-lysine-coated coverslips (MatTek) in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum.

Microscopic Technique
Fluorescence Microscopy, Fluorescence Resonance Energy Transfer (FRET)

Cell Type(s)
COS-1