Technical Reference #142

142.

Beta1-Adrenergic Receptor Association with PSD-95 Liaoyuan A. Hu; Yuting Tang; William E. Miller; Mei Cong; Anthony G. Laui; Robert J. Lefkowitz; and Randy A. Hall, Howard Hughes Medical Institute, The Journal fo Biological Chemistry, 275(142), (2000)
Link To Paper

Abstract:
The b1-adrenergic receptor (b1AR) is the most abundantsubtype of b-adrenergic receptor in the mammalian brainand is known to potently regulate synaptic plasticity. Tosearch for potential neuronal b1AR-interacting proteinswe screened a rat brain c

Materials & Methods:
Plasmids and Antibodies—Mammalian expression plasmids pcDNA3/Flag-b1AR and pcDNA3/Flag-b2AR were described before (39 40). b1AR-CT (the carboxyl-terminal 99 amino acids of the human b1AR) and b2AR-CT (the carboxyl-terminal 86 amino acids of the human b2AR) were amplified by PCR and then subcloned into pGEX-4T using the EcoRI and SalI restriction sites for GST fusion protein expression.

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
HEK-293