Technical Reference #105

105.

Laminin-Like Proteins Are Differentially Regulated During Cerebellar Development And Stimulate Granule Cell Neurite Outgrowth In Vitro. Powell; S.K.; Williams; C.O.; Nomizu; M.; Yamada; Y. and Kleinman. Laboratory of Developmental Biology; National Institute for Dental Research; National Institutes of Health; Bethesda; Maryland. Journal of Neuroscience Research; 54:-47 (998)., National Institutes of Health, The Journal of Neuroscience Research, 54(105), (1998)
Link To Paper

Abstract:
The basement membrane glycoprotein laminin-1 is a potent stimulator of neurite outgrowth. Although a variety of laminin isoforms have been described in recent years the role of alternative laminin isoforms in neural development remains largely uncharacterized. We found that a polyclonal antibody raised against the 1 1 and 1 chains of laminin-1 and a monoclonal antibody raised against the 2 chain of laminin-2 detect immunoreactive material in neuronal cell bodies in the developing mouse cerebellum. In addition laminin-1-like immunoreactivity was found in cell types throughout the cerebellum but laminin-2-like immunoreactivity was restricted to the Purkinje cells. Purified laminin-1 and laminin-2 stimulated neurite outgrowth in primary cultures of mouse cerebellar granule neurons to a similar extent whereas the synthetic peptides tested appeared to be active only for cell adhesion and not for stimulation of neurite outgrowth. The E8 proteolytic fragment of laminin-1 contained full neurite outgrowth activity. The identity of laminins expressed in granule neurons was also examined by Western blotting; laminin-like complexes were associated with the cell and appeared to have novel compositions. These results suggest that laminin-like complexes play important roles in cerebellar development. J. Neurosci. Res. 54:233-247 1998. © 1998 Wiley-Liss Inc. This article is a US Government work and as such is in the public domain in the United States of America.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Granule